Briefings in Functional Genomics and Proteomics Advance Access published online on December 17, 2008
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/eln053
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Comparative glycoproteomics: approaches and applications
Corresponding author. Dr Lingjun Li, School of Pharmacy, University of Wisconsin, 777 Highland Avenue, Madison, WI 53705-2222 USA. Tel: +1-608-265-8491; Fax: +1-608-262-5345; E-mail: lli{at}pharmacy.wisc.edu
Glycosylation plays fundamental roles in controlling various biological processes. Therefore, glycosylation analysis has become an important target for proteomic research and has great potential for clinical applications. With the continuous development and refinement of glycoprotein isolation methods, increasing attention has been directed to the quantitative and comparative aspects. This review describes the mass spectrometry (MS)-based techniques for the comparative analysis of glycoproteins and their applications to answer a wide range of interesting biological questions.
Keywords: biomarkers, glycoprotein quantitation, glycosylation, isotopic labelling, mass spectrometry, proteomics