Skip Navigation



Briefings in Functional Genomics and Proteomics Advance Access published online on November 21, 2008
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/eln046
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
7/6/454    most recent
eln046v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Moens, C. B.
Right arrow Articles by Ma, T. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moens, C. B.
Right arrow Articles by Ma, T. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. For permissions, please email: journals.permissions@oxfordjournals.org

Reverse genetics in zebrafish by TILLING

Cecilia B. Moens, Thomas M. Donn, Emma R. Wolf-Saxon and Taylur P. Ma

Corresponding author. Cecilia B. Moens, HHMI and Division of Basic Science, Fred Hutchinson Cancer Research Center, B2-152, 1100 Fairview Ave. N., Seattle, WA 98109, USA. Tel: +1 206 667 5627; Fax: +1 206 667 5939; E-mail: cmoens{at}fhcrc.org

TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Drosophila, Caenorhabditis elegans, rat, medaka and zebrafish and for the discovery of naturally occurring polymorphisms in humans. This review summarizes current TILLING methodologies as they have been applied to the zebrafish, ongoing TILLING projects and resources in the zebrafish community, and the future of zebrafish TILLING.

Keywords: zebrafish, TILLING, Cel1 mismatch cleavage, resequencing, reverse genetics


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
K. R. Monk, S. G. Naylor, T. D. Glenn, S. Mercurio, J. R. Perlin, C. Dominguez, C. B. Moens, and W. S. Talbot
A G Protein-Coupled Receptor Is Essential for Schwann Cells to Initiate Myelination
Science, September 11, 2009; 325(5946): 1402 - 1405.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.