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Briefings in Functional Genomics and Proteomics Advance Access published online on January 22, 2008
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/elm029
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© Oxford University Press, 2007, All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Genomics of mRNA turnover

José E. Pérez-Ortín

Corresponding author. José E. Pérez-Ortín, Departamento de Bioquímica y Biología Molecular, Facultad de Biológicas, Dr Moliner 50. E46100 Burjassot, Spain. Tel: +34 963 543467; Fax: +34 963 4635; E-mail: jose.e.perez{at}uv.es

Most studies on eukaryotic gene regulation have focused on mature mRNA levels. Nevertheless, the steady-state mRNA level is the result of two opposing biological processes: transcription and degradation, both of which can be important points to regulate gene expression. It is now possible to determine the transcription and degradation rates (TR and DR), as well as the mRNA amount, for each gene using DNA chip technologies. In this way, each individual contribution to gene expression can be analysed. This review will deal with the techniques used for the genomic evaluation of TR and DR developed for the yeast Saccharomyces cerevisiae. They will be described in detail and their potential drawbacks discussed. I will also discuss the integration of the data obtained to fully analyse the expression strategies used by yeast and other eukaryotic cells.

Keywords: run-on, macroarray, transcription rate, mRNA stability, ChIP-chip


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