Skip Navigation



Briefings in Functional Genomics and Proteomics Advance Access published online on November 22, 2006
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/ell034
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
5/4/249    most recent
ell034v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mitulovic, G.
Right arrow Articles by Mechtler, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mitulovic, G.
Right arrow Articles by Mechtler, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© Oxford University Press, 2006, All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Original Article

HPLC techniques for proteomics analysis—a short overview of latest developments

Goran Mitulovic and Karl Mechtler

Corresponding author. Goran Mitulovic, Research Institute of Molecular Pathology, Dr. Bohrgasse 7, A-1030 Wien, Austria. E-mail: mitulovic{at}imp.univie.ac.at

Due to the complex nature of the proteome, instrumentation and methods development for sample cleanup, fractionation, preconcentration, chromatographic separation and detection becomes urgent for the identification of peptides and proteins. Newly developed techniques and equipment for separation and detection, such as nano-HPLC and multidimensional HPLC for protein and peptide separation, enabled proteomics to experience dynamic growth during the past few years. In any proteomic analysis the most important and sometimes most difficult task is the separation of the complex mixture of proteins or peptides. This review describes some aspects and limitations of HPLC, both multidimensional and one-dimensional, in proteomics research without attempting to discuss all available HPLC methods, which would need far more space than available here.

Keywords: proteomics, liquid chromatography-mass spectrometry, multidimensional separation, post-translational modifications


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
U. Kruse, M. Bantscheff, G. Drewes, and C. Hopf
Chemical and Pathway Proteomics: Powerful Tools for Oncology Drug Discovery and Personalized Health Care
Mol. Cell. Proteomics, October 1, 2008; 7(10): 1887 - 1901.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.