Briefings in Functional Genomics and Proteomics Advance Access published online on May 23, 2006
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/ell025
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
* To whom correspondence should be addressed. The response of Desulfovibrio vulgaris Hildenborough (DvH), a sulphate-reducing bacterium, to nitrate stress was examined using quantitative proteomic analysis. DvH was stressed with 105 mM sodium nitrate (NaNO3), a level that caused a 50% inhibition in growth. The protein profile of stressed cells was compared with that of cells grown in the absence of nitrate using the iTRAQ peptide labelling strategy and tandem liquid chromatography separation coupled with mass spectrometry (quadrupole time-of-flight) detection. A total of 737 unique proteins were identified by two or more peptides, representing 22% of the total DvH proteome and spanning every functional category. The results indicate that this was a mild stress, as proteins involved in central metabolism and the sulphate reduction pathway were unperturbed. Proteins involved in the nitrate reduction pathway increased. Increases seen in transport systems for proline, glycine-betaine and glutamate indicate that the NaNO3 exposure led to both salt stress and nitrate stress. Up-regulation observed in oxidative stress response proteins (Rbr, RbO, etc.) and a large number of ABC transport systems as well as in iron-sulphur-cluster-containing proteins, however, appear to be specific to nitrate exposure. Finally, a number of hypothetical proteins were among the most significant changers, indicating that there may be unknown mechanisms initiated upon nitrate stress in DvH. Alyssa Redding is a graduate student in the Department of Chemical Engineering at the University of California at Berkeley. She is part of the Keasling laboratory and studies proteomics as part of the Genomics: GTL project. Aindrila Mukhopadhyay manages the Functional Genomics Core of the Genomics: GTL project. She is a staff scientist in the Physical Biosciences division of the Lawrence Berkeley National laboratory and a part of the Keasling research team. Dominique Joyner is a research associate at LBNL in the Ecology Department. Recieved a BS in Environmental Biology with a minor in Chemistry from CSU Northridge and a MS in Plant and Microbial Biology from UC Berkeley. Terry Hazen is Head of the Ecology Department and Center for Environmental Biotechnology at LBNL. He is a co-director of the Virtual Institute for Microbial Stress and Survival, and leads the Applied Environmental Biology Core of the Genomics: GTL project. Jay Keasling leads the Functional Genomics Core of the Genomics: GTL project. He is a Professor in the Departments of Chemical Engineering and Bioengineering at the University of California at Berkeley. He is also the head of the Synthetic Biology Department and the Physical Biosciences division at the Lawrence Berkeley National laboratory.
Original Article
Study of nitrate stress in Desulfovibrio vulgaris Hildenborough using iTRAQ proteomics
Alyssa M. Redding,
Aindrila Mukhopadhyay,
Dominique C. Joyner,
Terry C. Hazen,
and
Jay D. Keasling *
Jay D. Keasling, E-mail: keasling{at}berkeley.edu
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. M. R. Lacerda and K. F. Reardon Environmental proteomics: applications of proteome profiling in environmental microbiology and biotechnology Brief Funct Genomic Proteomic, March 11, 2009; (2009) elp005v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Bender, H.-C. B. Yen, C. L. Hemme, Z. Yang, Z. He, Q. He, J. Zhou, K. H. Huang, E. J. Alm, T. C. Hazen, et al. Analysis of a Ferric Uptake Regulator (Fur) Mutant of Desulfovibrio vulgaris Hildenborough Appl. Envir. Microbiol., September 1, 2007; 73(17): 5389 - 5400. [Abstract] [Full Text] [PDF]
|
|