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Briefings in Functional Genomics and Proteomics Advance Access published online on May 18, 2006
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/ell023
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© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions please email: journals.permissions@oxfordjournals.org

Original Article

Developments in mass spectrometry for the analysis of complex protein mixtures

Gurusahai Khalsa-Moyers and William Hayes McDonald *


  Abstract

State-of-the-art proteomics workflows involve multiple interdependent steps: sample preparation, protein-peptide separation, mass spectrometry and data analysis. While improvements in any of these steps can increase the depth and breadth of analysis, advances in mass spectrometry have catalysed many of the most important developments. We discuss common classes of mass analysers and how these analysers are put together to produce some of the most popular mass spectrometry platforms. The capabilities of these platforms determine how they can be used in a variety of common proteomic strategies and, in turn, what types of biological questions can be addressed. Moving forward, powerful new hybrid mass spectrometers and application of emerging types of tandem mass spectrometry promise that our ability to analyse complex mixtures of proteins will continue to advance.

Keywords: proteomics; technologies; mass spectrometers; tandem mass spectrometry.

Gurusahai Khalsa-Moyers is a PhD candidate in the Genome Science and Technology graduate program at the University of Tennessee, Knoxville, TN, USA.

W. Hayes McDonald is a staff scientist in the Chemical Sciences Division, Organic and Biological Mass Spectrometry Group at Oak Ridge National Laboratory, Oak Ridge, TN, USA.


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