Skip Navigation



Briefings in Functional Genomics and Proteomics Advance Access published online on May 10, 2006
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/ell017
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
5/2/169    most recent
ell017v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hood, B. L.
Right arrow Articles by Veenstra, T. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hood, B. L.
Right arrow Articles by Veenstra, T. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. All rights reserved. For permissions please email: journals.permissions@oxfordjournals.org

Technique Review

Unravelling the proteome of formalin-fixed paraffin-embedded tissue

Brian L. Hood, Thomas P. Conrads, and Timothy D. Veenstra *

* To whom correspondence should be addressed.
Timothy D. Veenstra, E-mail: veenstra{at}ncifcrf.gov


  Abstract

Biofluid detectable biomarkers that originate at the site of diseased tissues would be advantageous, in that, they may provide mechanistic information concerning the manifestation and progression of the disease. Unfortunately, tissue biopsies are precious samples that can generally be acquired in small amounts due to the invasive nature of the sample collection. One of the foundations of pathological diagnosis for decades has been from formalin-fixed paraffin-embedded (FFPE) tissues, of which a vast archive exists worldwide. These tissues have also been widely used for immunohistochemistry and in situ hybridization studies examining for expression of specific proteins or transcripts. Unfortunately, the ability to analyse FFPE tissues using mass spectrometry (MS) has been essentially non-existent until recently. In this review, methods that allow the extraction of peptides from FFPE tissues and their proteomic analysis using MS are described. The ability to identify the proteins extracted from FFPE tissues allows comparative analyses that enable the potential discovery of novel biomarkers at the site of the diseased tissue.

Keywords: biomarker discovery; formalin-fixed paraffin-embedded tissue; laser-capture microdissection; mass spectrometry; proteomics.

Brian Hood is a research scientist in the Laboratory of Proteomics and Analytical Technologies at SAIC-Frederick, Inc. He received his PhD in Biochemistry from The Ohio State University in 2003 under the direction of Dr Russ Hille.

Tom Conrads is the director of the Mass Spectrometry Center at SAIC-Frederick, Inc. He is keenly interested in utilizing mass spectrometry technology to aid in the diagnosis of human diseases.

Tim Veenstra is the director of the Laboratory of Proteomics and Analytical Technologies at SAIC-Frederick, Inc. His research interests lie in finding novel cancer-related biomarkers.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.