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Briefings in Functional Genomics and Proteomics Advance Access published online on February 27, 2006
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/ell012
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© Oxford University Press, 2006, All rights reserved. For permissions please email: journals.permissions@oxfordjournals.org

Technique Review

Mass spectrometry of RNA: linking the genome to the proteome

Zhaojing Meng and Patrick A. Limbach *

* To whom correspondence should be addressed.
Patrick A. Limbach, E-mail: Pat.Limbach{at}uc.edu


  Abstract

Ribonucleic acids (RNAs) are continuing to attract increased attention as they are found to play pivotal roles in biological systems. Just as genomics and proteomics have been enabled by the development of effective analytical techniques and instrumentation, the large-scale analysis of non-protein coding (nc)RNAs will benefit as new analytical methodologies, such as mass spectrometry (MS), are developed for their analysis. Mass spectrometry offers a number of advantages for RNA analysis arising from its ability to provide mass and sequence information starting with limited amounts of sample. This review will highlight recent developments in the field of MS that enable the characterization of RNA modification status, RNA tertiary structures, and ncRNA expression levels. These developments will also be placed in perspective of how MS of RNAs can help elucidate the link between the genome and proteome.

Keywords: Ribonucleic acid; post-transcriptional modifications; quantitation; mass spectrometry.

Zhaojing Meng is a postdoctoral fellow in the Laboratory of Proteomics and Analytical Technologies at SAIC-Frederick Inc., Frederick, MD. She received her PhD in Chemistry under the direction of Pat Limbach in 2004.

Pat Limbach is a Professor of Chemistry at the University of Cincinnati. He has a long-standing interest in RNA structure and characterization.

Submitted to Briefings in Functional Genomics and Proteomics as an invited Review Abstract.


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