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Briefings in Functional Genomics and Proteomics Advance Access published online on February 22, 2006
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/ell009
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© Oxford University Press, 2006, All rights reserved. For permissions please email: journals.permissions@oxfordjournals.org

Paper

Generation of multipurpose alleles for the functional analysis of the mouse genome

Frank Schnütgen *

* To whom correspondence should be addressed.
Frank Schnütgen, E-mail: schnuetgen{at}em.uni-frankfurt.de


  Abstract

A novel generation of retroviral gene-trap vectors has been developed with the ability to induce conditional mutations in most genes expressed in mouse embryonic stem (ES) cells. The vectors rely on directional site-specific recombination systems, which can repair and re-induce the gene-trap mutations when activated in succession. After the gene-trap insertions are passaged into mice, this system enables the induction of temporally and spatially restricted mutations in somatic cells. In addition to their conditional features, the vectors create multipurpose alleles amenable to a wide range of post-insertional modifications. These vectors have been used to assemble the largest library of ES cell lines with conditional mutations in single genes, presently totalling 1724 unique genes. Due to their efficiency, the vectors are part of the core technologies to be used by EUCOMM for establishing a complete collection of conditional null mutations in mice.

Keywords: gene trap; Cre recombinase; FLPe recombinase; recombination; conditional; cassette exchange.

Frank Schnütgen leads the vector development group in Harald von Melchner's laboratory and is a member of the German Gene Trap Consortium.


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