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Briefings in Functional Genomics and Proteomics Advance Access published online on February 23, 2006
Briefings in Functional Genomics and Proteomics, doi:10.1093/bfgp/ell007
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© Oxford University Press, 2006, All rights reserved. For permissions please email: journals.permissions@oxfordjournals.org

Paper

Cellular phenotyping by RNAi

Florian Fuchs and Michael Boutros *

* To whom correspondence should be addressed.
Michael Boutros, E-mail: m.boutros{at}dkfz.de


  Abstract

A systematic characterization of genes with unknown function is a key challenge after the sequencing of the human genome and the genomes of many model organisms. High-throughput RNA-interference (RNAi) screenings have become a widely used approach in invertebrate model organisms and also promise to revolutionize cell biology in mammals. Genome-wide RNAi screens in Caenorhabditis elegans and Drosophila, and in a smaller scale in mammalian cells have proven to be a valuable and successful method for the dissection of diverse biological processes. A number of RNAi libraries have become available that rely on different technologies, such as long double-stranded (ds) RNAs, in vitro diced short-interfering (si) RNAs, synthetic siRNAs and short-hairpin (sh) RNAs, which all have specific advantages and disadvantages. In addition, progress in screening technologies and data analysis allows the adaptation of screening methods to analyse more complex cellular processes. This review will summarize strategies in combining genome-scale RNAi libraries, high-throughput screening technologies, integrated high-content data analysis and will discuss future challenges.

Keywords: RNAi screening; functional genomics; high-throughput cell-based assays.

Florian Fuchs and Michael Boutros are members of the Boveri-Group on Signaling and Functional Genomics at the German Cancer Research Center.


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