Skip Navigation


Briefings in Functional Genomics and Proteomics Advance Access originally published online on May 10, 2006
Briefings in Functional Genomics and Proteomics 2006 5(2):112-120; doi:10.1093/bfgp/ell018
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
5/2/112    most recent
ell018v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Aggarwal, K.
Right arrow Articles by Lee, K. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aggarwal, K.
Right arrow Articles by Lee, K. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© Oxford University Press, 2006, All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Special Issue Papers

Shotgun proteomics using the iTRAQ isobaric tags

Kunal Aggarwal, Leila H. Choe and Kelvin H. Lee

Corresponding author. Kelvin H. Lee, School of Chemical and Biomolecular Engineering, Cornell University, 120 Olin Hall, Ithaca, NY 14853-5201, USA. E-mail: KHL9{at}cornell.edu

Shotgun proteomic methods involving isobaric tagging of peptides enable high-throughput proteomic analysis. iTRAQ reagents allow simultaneous identification and quantitation of proteins in four different samples using tandem mass spectrometry (MS). In this article, we provide a brief description of proteome analysis using iTRAQ reagents and review the current applications of these reagents in proteomic studies. We also compare different aspects of protein identification including protein sequence coverage and proteome coverage obtained using iTRAQ reagents with those using other shotgun proteomic techniques. We briefly discuss the issue of isotope purity correction in measured peak areas during protein quantitation using iTRAQ reagents. Finally, we conclude with some of the current challenges in MS-based proteomic analysis that are limiting protein identifications obtained by different shotgun proteomic methods.

Keywords: isobaric tag, iTRAQ, shotgun proteomics, tandem mass spectrometry


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
Y. Liu, B. K. Law, and H. Luesch
Apratoxin A Reversibly Inhibits the Secretory Pathway by Preventing Cotranslational Translocation
Mol. Pharmacol., July 1, 2009; 76(1): 91 - 104.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. P. Mirza and M. Olivier
Methods and approaches for the comprehensive characterization and quantification of cellular proteomes using mass spectrometry
Physiol Genomics, October 8, 2008; 33(1): 3 - 11.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
N. J. Lehrbach and E. A. Miska
Functional genomic, computational and proteomic analysis of C. elegans microRNAs
Brief Funct Genomic Proteomic, June 19, 2008; (2008) eln024v1.
[Abstract] [Full Text] [PDF]

Home page
 Brief BioinformHome page
B.-J. M. Webb-Robertson and W. R. Cannon
Current trends in computational inference from mass spectrometry-based proteomics
Brief Bioinform, September 1, 2007; 8(5): 304 - 317.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. J. Radosevich, T. A. Reinhardt, J. D. Lippolis, J. P. Bannantine, and J. R. Stabel
Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187
J. Bacteriol., February 1, 2007; 189(3): 1109 - 1117.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.