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Briefings in Functional Genomics Advance Access published online on December 1, 2009
Briefings in Functional Genomics, doi:10.1093/bfgp/elp047
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© The Author 2009. Published by Oxford University Press. For permissions, please email: journals.permissions@oxfordjournals.org

Quantitative cell array screening to identify regulators of gene expression

Pinay Kainth and Brenda Andrews

Corresponding author. Brenda J. Andrews. Terrence Donnelly Center for Cellular and Biomolecular Research, Banting and Best Department of Medical Research, Department of Molecular Genetics, University of Toronto, 160 College Street, Toronto, Ontario, Canada, M5S 3E1. Tel: +1-416-978-8562; Fax: +1-416-946-8253; E-mail: brenda.andrews{at}utoronto.ca

In the last decade or so, advances in genome-scale technologies have allowed systematic and detailed analysis of gene function. The experimental accessibility of budding yeast makes it a test-bed for technology development and application of new functional genomic tools and resources that pave the way for comparable efforts in higher eukaryotes. In this article, we review advances in reporter screening technology to discover trans-acting regulators of promoters (or cis-elements) of interest in the context of a novel functional genomics approach called Reporter Synthetic Genetic Array (R-SGA) analysis. We anticipate that this methodology will enable researchers to collect quantitative data on hundreds of gene expression pathways in an effort to better understand transcriptional regulatory networks.

Keywords: gene expression, reporter gene, Saccharomyces cerevisiae, cell cycle, histone gene


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