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Briefings in Functional Genomics Advance Access published online on January 24, 2008
Briefings in Functional Genomics, doi:10.1093/bfgp/elm035
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© Oxford University Press, 2008. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Improving yeast two-hybrid screening systems

Manfred Koegl and Peter Uetz

Corresponding author. Dr M. Koegl, Translational Research, and Genomics and Proteomics Core Facilities, German Cancer Research Center, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany. Tel: +49 6221 42 47 10; Fax: +49 6221 42 47 04; E-mail: m.koegl{at}dkfz.de

Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of protein–protein interactions. Optimisation and automation has increased the throughput of the method to an extent that allows the systematic mapping of protein–protein interactions on a proteome-wide scale. Since two-hybrid screens fail to detect a great number of interactions, parallel high-throughput approaches are needed for proteome-wide interaction screens. In this review, we discuss and compare different approaches for adaptation of Y2H screening to high-throughput, the limits of the method and possible alternative approaches to complement the mapping of organism-wide protein–protein interactions.

Keywords: Yeast two-hybrid, protein–protein interactions, interaction proteome


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