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Briefings in Functional Genomics Advance Access originally published online on October 31, 2008
Briefings in Functional Genomics 2008 7(6):427-443; doi:10.1093/bfgp/eln038
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Using retroviruses as a mutagenesis tool to explore the zebrafish genome

Li-En Jao, Lisette Maddison, Wenbiao Chen and Shawn M. Burgess

Corresponding author. Shawn M. Burgess, Bldg. 50 Rm. 5537, 50 South Dr., Bethesda, MD 20892, USA. Tel: +1 301 594 8224; E-mail: burgess{at}mail.nih.gov

We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms.

Keywords: genetics, moloney murine leukemia virus, enhancer traps, gene traps, linker-mediated PCR, zebrafish


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