Skip Navigation

Briefings in Functional Genomics 2006 5(2):154-168; doi:10.1093/bfgp/ell024
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nunn, B. L.
Right arrow Articles by Goodlett, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nunn, B. L.
Right arrow Articles by Goodlett, D. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© Oxford University Press, 2006, All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Special Issue Papers

Comparison of a Salmonella typhimurium proteome defined by shotgun proteomics directly on an LTQ-FT and by proteome pre-fractionation on an LCQ-DUO

Brook L. Nunn, Scott A. Shaffer, Alexander Scherl, Byron Gallis, Manhong Wu, Samuel I. Miller and David R. Goodlett

Corresponding author. David R. Goodlett, Medicinal Chemistry Department, University of Washington, Box 335351, Seattle, WA 98195, USA. E-mail: goodlett{at}u.washington.edu

Shotgun proteomics is rapidly becoming one of the most efficient and popular tools to examine protein expression in cells. Numerous laboratories now have a wide array of low- and high-performance mass spectrometry instrumentation necessary to complete proteome-wide projects. Often these laboratories have time and financial constraints that prohibit all projects from being conducted on high-performance state-of-the-art mass spectrometers. Here, we compare shotgun proteomic results using a direct ‘lyse, digest and analyse’ approach on a high-performance mass spectrometer (i.e. the LTQ-FT) with the results from a much lower-performance instrument (i.e. the LCQ-DUO) where, for the latter, various traditional protein pre-fractionation steps and gas-phase fractionation were used to increase the proteome coverage. Our results demonstrate that shotgun proteomic analyses conducted on the lower-performance LCQ-DUO mass spectrometer could adequately characterize a PhoP constitutive strain of Salmonella typhimurium if proteome pre-fractionation steps and gas-phase fractionation were included.

Keywords: shotgun proteomics, ion trap mass spectrometer, Salmonella, linear ion trap and Fourier transform ion cyclotron resonance mass spectrometer, membrane proteome


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Brief Funct Genomic ProteomicHome page
D. Martins de Souza, B. M. Oliveira, E. Castro-Dias, F. V. Winck, R. S. O. Horiuchi, P. A. Baldasso, H. T. Caetano, N. K. D. Pires, S. Marangoni, and J. C. Novello
The untiring search for the most complete proteome representation: reviewing the methods
Briefings in Functional Genomics, July 1, 2008; 7(4): 312 - 321.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.